Insertion junction: CLIP2.065197_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065197
Chromosome:chromosome 1
Location:4077691
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g027000 RPL11 (1 of 1) K02868 - large subunit ribosomal protein L11e (RP-L11e, RPL11); Cytosolic 80S ribosomal protein L11 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAAGTCCTGCAGCTAATGGCACCAACACAGTCCAAACTGAAACAACAGC
Internal bar code:TTGGCAAGAATAGCAGTTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1129
LEAP-Seq percent confirming:94.5455
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGATGCTTACGGGGCA
Suggested primer 2:GGCTGTGCGTTGACATTGTT