Insertion junction: CLIP2.065201_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065201
Chromosome:chromosome 11
Location:2056073
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g801290 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCGGGCATACAATGATACATGCAACCAGGCCATCTCGATGCTTGTTCC
Internal bar code:TTAATCGAGTCGCAGACGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1532
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTGAGTGTTGCTGGCAG
Suggested primer 2:ACCCCCACAATCGGTCAATC