| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065202 |
| Chromosome: | chromosome 16 |
| Location: | 5539009 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCAGGGCATCCCCGGGACACCAAGTAAAAATTCGAAGGCCCTGCAT |
| Internal bar code: | GGTTGGGCAATCGGGCATCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCCGTGGTCAATTGCTA |
| Suggested primer 2: | ACTCGATGGTGCAAAGAGGG |