| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065216 |
| Chromosome: | chromosome 10 |
| Location: | 4894115 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453700 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAGCACACGTGTACCACTCCGGCAAGTCCCTAGGGACTGCGTGAGGC |
| Internal bar code: | AATCGCTGAATTTTGTAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGACGAGGAGTATGAGCG |
| Suggested primer 2: | TGATGACCCGTGTCTTTGCA |