| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065243 |
| Chromosome: | chromosome 10 |
| Location: | 3053016 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441000 | (1 of 1) K17498 - transcription factor SPN1 (SPN1, IWS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACCGACCTGTGTCCCTACCGTAAAACTGGAAACCAGAAGCCCAGCAC |
| Internal bar code: | GGGGATCGAGTCTCTACCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5566 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGCTCGACTATCCACC |
| Suggested primer 2: | TCCGAGGTGTTGTTTCCGAG |