| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065251 |
| Chromosome: | chromosome 1 |
| Location: | 4015427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTAGACCCCACGCTCCGCCGCCAAACTGCGGCACCAGGGGCGATCA |
| Internal bar code: | TGACAGATTCGCTTTGGCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6512 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTAGTGTGTCGTCCTGC |
| Suggested primer 2: | CAGAAGCTGCACCAGGTGTA |