Insertion junction: CLIP2.065254_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.065254
Chromosome:chromosome 17
Location:294630
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697934 ATP23 Assembly factor for F1 mitochondrial ATP synthase; (1 of 1) K18156 - mitochondrial inner membrane protease ATP23 [EC:3.4.24.-] (ATP23, XRCC6BP1) 5'UTR
Cre17.g697950 AAA3 Plastidic ADP/ATP translocase; (1 of 2) PTHR31187:SF1 - ADP,ATP CARRIER PROTEIN 1, CHLOROPLASTIC-RELATED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTGCGTTGCTGGTGCAGCGTGGGGTAAATTTATTACTCGCGGCAATT
Internal bar code:CGATACTATCGGCATACTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1499
LEAP-Seq percent confirming:70.3704
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAGCGAGAAAAGATCCC
Suggested primer 2:CTTTCAAAAACGCGAGCCCA