| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065257 |
| Chromosome: | chromosome 10 |
| Location: | 1351880 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427700 | HEL47 | (1 of 1) PTHR24031:SF235 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 37-RELATED; DEAD box ATP-dependent RNA helicase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATGTGTGGATACGCTCCTCCTAGCTGGACACTCGTCTCAGCTGGACA |
| Internal bar code: | TAGATCGCGTACACTCCCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4203 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGCTGTTGGGAAAGTG |
| Suggested primer 2: | CAACCGACAGCGCAAAAGAA |