Insertion junction: CLIP2.065268_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.065268
Chromosome:chromosome 6
Location:3037429
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g274650 NUOAF4 (1 of 3) IPR008979//IPR013857 - Galactose-binding domain-like // NADH:ubiquinone oxidoreductase intermediate-associated protein 30; Complex I intermediate-associated CIA30 protein, mitochondrial 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGAACCCACGATCCAACTCTCTCAGCCACCGCTCTGTCCGCGGCGCTG
Internal bar code:GTTCGGTACACCTTCAGTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:97.619
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAGGAATAACGAGGCGCT
Suggested primer 2:GAGACTCCAGATCGTCGCAG