Insertion junction: CLIP2.065272_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.065272
Chromosome:chromosome 7
Location:5594776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g800857 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAAGCTACCGCGAGCGAGAGGCCCTATTGCCCCGTCTGGCAGTCACAA
Internal bar code:TGAATGTACTTGTCAATTCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCGGGCATGAGATGAGG
Suggested primer 2:GTTGCAAGAAAGCGTTCCGT