| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065273 |
| Chromosome: | chromosome 13 |
| Location: | 4174477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591350 | MFT27 | Major facilitator superfamily transporter; (1 of 22) IPR011701//IPR020846 - Major facilitator superfamily // Major facilitator superfamily domain | 5'UTR |
| Cre13.g591400 | COA1,COAA1 | (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAGCTATGCCATTGGTCCTTGTTCCAAAGTTTGTTGGTTATCCAGTT |
| Internal bar code: | TTGCAGACACGATTTACACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1792 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGATGTTTAGCCACCGT |
| Suggested primer 2: | GCATGGAAGACGGGGATAGG |