| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065290 |
| Chromosome: | chromosome 3 |
| Location: | 4738292 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177550 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGGCTCACTTGCACTCCCGCCATCCTTCACAACGCTCAGGCGCCCAG |
| Internal bar code: | ACGCGGAATTGTCGAACTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1099 |
| LEAP-Seq percent confirming: | 41.1765 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGATGTGCTGGGATCA |
| Suggested primer 2: | CTACAACAGGGCAAGCCTGA |