| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065299 |
| Chromosome: | chromosome 3 |
| Location: | 5378846 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183500 | (1 of 1) K14851 - ribosomal RNA-processing protein 17 (RRP17, NOL12) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGACGACAGCGCAACGTTGTGATATGGCCGTATGGCATGGGGTGCGCA |
| Internal bar code: | TCCATGTTCTTGAGATTAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2137 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTTCTCCCTTCCGAAA |
| Suggested primer 2: | AAGAAGGGGAAGCGGTGATG |