Insertion junction: CLIP2.065312_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.065312
Chromosome:chromosome 16
Location:4626976
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670750 (1 of 1) IPR012312//IPR012827 - Haemerythrin-like // Haemerythrin, metal-binding domain 3'UTR
Cre16.g670754 (1 of 1) PTHR10217//PTHR10217:SF512 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTTTCCTACTTTCCTACATGCCCGACCATGCTGCCAACCAGGCTGAC
Internal bar code:CCTGTCGACAGGAATGCCTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:884
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGAAACAGAGACGGGTG
Suggested primer 2:GGGTTTGGGGCGAATTCCTA