| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065337 |
| Chromosome: | chromosome 7 |
| Location: | 6042180 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800867 | (1 of 3) PF09011 - HMG-box domain (HMG_box_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAATATGTTACAGGGAGAGGGGTTTCCACCCTATCAGTCCGCATATG |
| Internal bar code: | GGCAGAGCGGATTAAGGTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3300 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGACCTACAAGCCCCAT |
| Suggested primer 2: | CTGCTACATGCTGTGGGTGA |