| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065343 |
| Chromosome: | chromosome 2 |
| Location: | 7464263 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800263 | 5'UTR | ||
| Cre02.g800264 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTGGGGTGAGTGACGGCGGGCACGCGTGCGGCGTGCCGACGCGCTAC |
| Internal bar code: | GTGAGCTCATATCTCTTCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGAGTAGCAGCTACGGC |
| Suggested primer 2: | ATCACGAAGGCGGAGTTTGT |