Insertion junction: CLIP2.065346_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.065346
Chromosome:chromosome 15
Location:1211925
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801719 NCL59 Nuclear Control of chloroplast-Like 59; (1 of 5) IPR013584//IPR016024 - RAP domain // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATGTTAGCCTCGTGCTGGCTGTGCTGCCTCCACACATCAGGAAGAATA
Internal bar code:TGAGACGGGGCGGCTAGTGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:830
LEAP-Seq percent confirming:64.7059
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGTGGTGATGGTAGTGG
Suggested primer 2:CATTCTCGCGCCTGACAATG