| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.065352 |
| Chromosome: | chromosome 9 |
| Location: | 391364 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCCTCGGCCCGGTAGCCCTCCACCTCGCGGTTGAGAGACGACATCA |
| Internal bar code: | ACTGATGATTCGGGACGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 71 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGTGCGTGTGTATGG |
| Suggested primer 2: | CCCCACAGTCAGTTGGGTTT |