Insertion junction: CLIP2.065356_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065356
Chromosome:chromosome 1
Location:7662326
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g053700 (1 of 7) PF00027//PF00520 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion transport protein (Ion_trans) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGCATAATGTGACCATGCACGACGTCACAATTCGCGCTTCTCTCATG
Internal bar code:CGGGAGGGGCGAGTCACCCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2236
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTGATGAGGGGTGTGTGCAT
Suggested primer 2:GGCTCGGTTGCAACAATGTT