| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065367 |
| Chromosome: | chromosome 14 |
| Location: | 2523569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624500 | RRP46 | Exosome complex exonuclease component; (1 of 1) K12590 - exosome complex component RRP46 (RRP46, EXOSC5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCGCAACGCGGAAGCGGCTGGGCGTTGAGCAGGGGTCTTGTAGGCG |
| Internal bar code: | GACGATGGGAAGGGCTATTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1984 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATGGCCAAACCTCTCAT |
| Suggested primer 2: | CATCATGTTTAGGGCGCGTG |