| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.065369 |
| Chromosome: | chromosome 13 |
| Location: | 577093 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565450 | PURB,PURB1 | Adenylosuccinate lyase; (1 of 1) K01756 - adenylosuccinate lyase (purB, ADSL) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGTGTTTCCAAAACCGGGTACTAGTACCCATTCGGGTACTGAGAAAA |
| Internal bar code: | AAGGCCGGATTAGACGCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1248 |
| LEAP-Seq percent confirming: | 78.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGCGGGATACAGACTC |
| Suggested primer 2: | CTTTGCTCGCGGTGAATACG |