| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065378 |
| Chromosome: | chromosome 16 |
| Location: | 4493996 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g667700 | (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTTGACGCCCCCAAGCACACGCCCCACCACACGCGCCCCACCACCCA |
| Internal bar code: | ATTGCCGTTGACCCTTCATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACCT |
| Suggested primer 2: | ATGCTCGGGTTCTGGTCTTG |