| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065388 |
| Chromosome: | chromosome 10 |
| Location: | 2024101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432400 | OPR46 | OctotricoPeptide Repeat protein 46 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGAGGTTGTGAAGGGTGGTAATGAACAGTGACCTCAGCAATGCGGA |
| Internal bar code: | CCCCTGGTACCGATTGTACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2202 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGAGGGAAACGTGGC |
| Suggested primer 2: | TGTACGATGAGCGGCTAACC |