| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065389 |
| Chromosome: | chromosome 13 |
| Location: | 5099597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606800 | (1 of 2) PTHR30336//PTHR30336:SF4 - INNER MEMBRANE PROTEIN, PROBABLE PERMEASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACGTCGCGCCCAAGAGATGCATCCGTGTCCCAAGCCCAGACATGTAG |
| Internal bar code: | TGCGCGTGAAGTATAATAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2437 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACCGCTTTTTCTGCCTG |
| Suggested primer 2: | AAGCAACCACGTATCACCGT |