Insertion junction: CLIP2.065390_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.065390
Chromosome:chromosome 2
Location:7327254
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391615 (1 of 80) IPR003882 - Pistil-specific extensin-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAAACCCATCCAGCCAGCCACCTTTCTTATGTGGCGTACTCGGCTGTG
Internal bar code:ATGTTGGCGATGAATGTGAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:104
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTGAACCGAATGCCATC
Suggested primer 2:CGTCACCAAAGTCGAGGTCA