| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065394 |
| Chromosome: | chromosome 10 |
| Location: | 5954234 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCAAACCTGGTCGGCAAGCCCCGAGACCAGGTGCAATCAGTATGAAT |
| Internal bar code: | GCTTGTGATTAGACGGTTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3900 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATTGAAAGGCCTCGTGGG |
| Suggested primer 2: | CCCCCGATATGCACTCTGTC |