| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065405 |
| Chromosome: | chromosome 13 |
| Location: | 1855931 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575300 | (1 of 1) K08876 - SCY1-like protein 1 (SCYL1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACTGTCATGAAGCTGCACCATCAACCTGCGTACCGGTCTACCTCC |
| Internal bar code: | CAAAATCATTGAGAGGAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3457 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCACCTTTGCCGAGGAG |
| Suggested primer 2: | CCCCTGTATGGAAACTGCGT |