| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065425 |
| Chromosome: | chromosome 16 |
| Location: | 830182 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801884 | (1 of 1) K19001 - ATP-dependent DNA helicase (HELLS, DDM1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAGGTGAGCGCGTGTGTATGCGTTCCAAGGTCGCGGATGTTGGCGGC |
| Internal bar code: | ATTCAACAGACCAATTTCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1607 |
| LEAP-Seq percent confirming: | 93.1034 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAGGAGGAGAAGGAGG |
| Suggested primer 2: | AACAATCCCCGGTCCATCAC |