| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065434 |
| Chromosome: | chromosome 3 |
| Location: | 631674 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145547 | CBR1 | (1 of 3) K00326 - cytochrome-b5 reductase (E1.6.2.2); NADH-cytochrome b5 reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTACATAACGCTGGCAGCGTTACAGGGCCGCTTGCGTTGCAATGGTAT |
| Internal bar code: | TAGCGCAGGTCGCCAATGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2817 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGGGATGTTGCAGACT |
| Suggested primer 2: | CGAGGACAAGCAGTTCCAGT |