| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065437 |
| Chromosome: | chromosome 5 |
| Location: | 54455 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241750 | PDK3 | Pyruvate dehydrogenase kinase; (1 of 2) 2.7.11.2 - [Pyruvate dehydrogenase (acetyl-transferring)] kinase / Pyruvate dehydrogenase kinase (phosphorylating) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCCGGAACGACTCTACGTACCAGTCCCGCACCTAGCCACACCGCACA |
| Internal bar code: | ACAATGGCACCCCATTTCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCCAAGAAGCACATACT |
| Suggested primer 2: | CTACAGGCAGACGTGTGTGT |