Insertion junction: CLIP2.065451_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.065451
Chromosome:chromosome 1
Location:2805457
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g017200 FKB24,FKB99 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PTHR10516//PTHR10516:SF290 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE 5'UTR
Cre01.g017250 FKB99 Chlorophyceae-specific protein; (1 of 2) K09571 - FK506-binding protein 4/5 (FKBP4_5) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAGCAATGACATAGTGTCATGAGTGCACTGCTAGAAAGGACCCACTGAA
Internal bar code:TATCAAGTCCTGTAACATTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:737
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCTGTATGGCGATCTCC
Suggested primer 2:AACCGGTCCACACTCATGTC