| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065453 |
| Chromosome: | chromosome 10 |
| Location: | 6377910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464200 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | 5'UTR | |
| Cre10.g464250 | (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAGTGTAGATAAATGTTGCGTTGCTGTATTTTTTCATGTGCACGCTC |
| Internal bar code: | CATCGACGGTTGAGCGAGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2297 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGAAGCAGCTGCAAGG |
| Suggested primer 2: | TGGGAATGGGAGGCAGGATA |