Insertion junction: CLIP2.065466_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.065466
Chromosome:chromosome 6
Location:5597269
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g287400 STPK23,STK23 Serine/threonine protein kinase; (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTATAAGAGATGAATTGTCCACCAAATTATCTTCAAAAGTAGTACAAC
Internal bar code:GTATAAAAGGGCTAAATAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1608
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCATCACAATTAGGCGC
Suggested primer 2:CCACACACACAAAGCTCGTG