Insertion junction: CLIP2.065473_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.065473
Chromosome:chromosome 10
Location:1938489
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g431800 ARS5 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAGCGGGCCTGAGGACACGGGCCGGGAATCCGGTCCCCGACCCAATTA
Internal bar code:GAGGCCGGGTAATATCGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1395
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTCGAATCTGGGAGAGC
Suggested primer 2:CCGACCACCACCAACTACAA