| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.065478 |
| Chromosome: | chromosome 2 |
| Location: | 1876220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087450 | (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACAGGGGAGCGCACTTGTAAGCCTTGTGTGCGCGGCATGAGCATCACG |
| Internal bar code: | TGGGCATTCAATCGAGAAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1760 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCAAGCAAACACCAGC |
| Suggested primer 2: | TGACCCGTCACAACTTACCG |