| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065483 |
| Chromosome: | chromosome 10 |
| Location: | 3019994 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440650 | (1 of 1) PTHR12176:SF18 - METHYLTRANSFERASE-LIKE PROTEIN 13 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCTGCCCAGTTGTCCATGTCAACCTTGGGTGCGTACACCGCTGGCCG |
| Internal bar code: | TGGTTAACACTTCTGTACGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4792 |
| LEAP-Seq percent confirming: | 96.5318 |
| LEAP-Seq n confirming: | 167 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 173 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGCATCAGCTACGAGGC |
| Suggested primer 2: | GATTGCTGTGGTGTGTGCTG |