| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.065494 |
| Chromosome: | chromosome 1 |
| Location: | 50489 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000200 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain; Esterase, SGNH hydrolase-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACACGGCCTTCTCATCTTCTCCTGGTCTCGGAGTTTACCGGGGCCCT |
| Internal bar code: | TCCTTCTCACGAGGTTTTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTGACAAGCTGGCAGG |
| Suggested primer 2: | GTAATGGTGCGCGGAATCAC |