Insertion junction: CLIP2.065495_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065495
Chromosome:chromosome 3
Location:8626140
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g210177 (1 of 3) PF13405//PF13499 - EF-hand domain (EF-hand_6) // EF-hand domain pair (EF-hand_7) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTGTCATTCACGCGATAAGGCTGCGACGTAACGACCTTACGGCGCGA
Internal bar code:GATTGCTTACATTCTTGAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:63.6364
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAAATCCCCCTTCTTCCA
Suggested primer 2:GATCGTGTGCACCACCTGTA