| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065502 |
| Chromosome: | chromosome 6 |
| Location: | 3698740 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278153 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR | |
| Cre06.g278154 | (1 of 1) PTHR31560:SF0 - UPF0652 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTACGTATGGGCGTCTCAGACCCTGCAAAATTGAGCAATCCAGCGC |
| Internal bar code: | TAAACGTCTGGGGGGACAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5262 |
| LEAP-Seq percent confirming: | 99.5413 |
| LEAP-Seq n confirming: | 217 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 218 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGTTGCAGTGCTGTGTC |
| Suggested primer 2: | ATCAGTAATCGGAGGCAGCG |