| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065526 |
| Chromosome: | chromosome 10 |
| Location: | 293116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419800 | PIS1 | (1 of 1) K00999 - CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT); Phosphatidylinositol synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACTGACCGCGAGCGCGGTACTGTTTCGACTCAAACACTTCACTTAGA |
| Internal bar code: | TCTTTATGTAAGCTATTACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 2.7027 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAGTTGCGGTCCCTATG |
| Suggested primer 2: | GAGACCTACCGTATGCAGCC |