| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065532 |
| Chromosome: | chromosome 1 |
| Location: | 3876830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025450 | DRC9,FAP122 | Nexin-dynein regulatory complex 9; (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | 5'UTR |
| Cre01.g025500 | (1 of 1) PTHR19959//PTHR19959:SF135 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCTGAACTCGGCTATGGAGCGCCTTTGCACATTGAATCAGGCGGCA |
| Internal bar code: | AGGGGCGATCGATTAGCTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2462 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTCTGCCTGCTTTGTTG |
| Suggested primer 2: | AAAGACACCCCCAGCAAACA |