| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.065545 |
| Chromosome: | chromosome 10 |
| Location: | 6608757 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801217 | (1 of 39) PTHR13078:SF53 - PROTEIN MAOC-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTCGGTAACGTGAAAGAGGAAGGCACGGGAAGTGATGAAACAGAGCA |
| Internal bar code: | GTTTGCGCCGGAAAAAGATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1402 |
| LEAP-Seq percent confirming: | 96.0784 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTTGACAACTGTGCTG |
| Suggested primer 2: | TGGTTTTGGGACTGCACTGT |