| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065549 |
| Chromosome: | chromosome 8 |
| Location: | 3844828 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381600 | (1 of 1) IPR000104//IPR007112//IPR009009 - Antifreeze protein, type I // Expansin/pollen allergen, DPBB domain // RlpA-like double-psi beta-barrel domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGAATGTAACCCCCGACCCCCACTGCTGCTCCCTTACCCCGCCTAA |
| Internal bar code: | TGGGTCCGATATGGGGTATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGAGTCGCCCGAGAGTG |
| Suggested primer 2: | CCTTCACGCTAGCAACTCCA |