Insertion junction: CLIP2.065551_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.065551
Chromosome:chromosome 9
Location:1574197
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399000 SDR18 Short-chain dehydrogenase/reductase; (1 of 5) IPR002198//IPR002347//IPR003560//IPR013968 - Short-chain dehydrogenase/reductase SDR // Glucose/ribitol dehydrogenase // 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase // Polyketide synthase, ketoreductase domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTTGTGCTTGGACTGCGGCCACCTCCTCCATTACCCCCCACCCCCTC
Internal bar code:AGTTAGTATTGAGGCTAATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1463
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCTCCTGTGTGTCTCC
Suggested primer 2:AAGGGGACTAGCTCCGTGAT