| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065553 |
| Chromosome: | chromosome 3 |
| Location: | 6766557 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196600 | RAD17 | (1 of 1) PTHR12172//PTHR12172:SF0 - CELL CYCLE CHECKPOINT PROTEIN RAD17 // RAD17; presequence translocase-associated protein import motor subunit | 3'UTR |
| Cre03.g196651 | RAD10 | DNA repair protein; (1 of 1) K10849 - DNA excision repair protein ERCC-1 (ERCC1) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTCTGACGTGGCAGCAGGCTGGCGTCCTTCGTCCTAGTGCTCTGGTG |
| Internal bar code: | ATGTTATCTTCGCTACAGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2048 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCATGAGCCGTTCAGGA |
| Suggested primer 2: | AGCTACTTCTGGAACGGTGC |