| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065569 |
| Chromosome: | mating type plus |
| Location: | 363391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254224 | SRL1B,SRLb | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGACACGTACGCACACTCGAGGAGGGAACCTCGAATGACCCCCATGCT |
| Internal bar code: | CTTGTGGGAGCGAGAGCATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3813 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCTGTGCTAGGGTGTTG |
| Suggested primer 2: | GTTTAGGGTGGTTTGCGCTG |