Insertion junction: CLIP2.065585_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.065585
Chromosome:chromosome 7
Location:4396250
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g340850 CYP743A2,CYP19 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTCCTCCTACGGCCGCGTGCTGGTGCAGGCGTGCCGGGACGTGTTCA
Internal bar code:GCATTACGGGTATCGAGGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GACACTCACGGTCTTGAGCA
Suggested primer 2:CCTAAGGCCTCACACCCATC