Insertion junction: CLIP2.065586_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.065586
Chromosome:chromosome 11
Location:4373461
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g482900 RAB20,RAB18C,RABC3 Small Rab-related GTPase; (1 of 3) K07910 - Ras-related protein Rab-18 (RAB18) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTCGCTAGCGTGTCATCGCGATGGTCGATACACATGACTATACATTC
Internal bar code:TTACGAAAGGTTGTAAGTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2976
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACTGTCAACTTGCAGC
Suggested primer 2:TGCAACGTAACCAAACAGCG