| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065590 |
| Chromosome: | chromosome 13 |
| Location: | 3251996 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801550 | (1 of 1) K11793 - cereblon (CRBN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACGTGCTGTCGTCGTTTCGTGTCAGAGGTTCCGTGTTGTGTCTTTGT |
| Internal bar code: | AGTATATTTCTCACCTTTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1205 |
| LEAP-Seq percent confirming: | 52.6316 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCAATGGGGAGGAAGT |
| Suggested primer 2: | GCGTGGACTATGATGGGGAG |