| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065594 |
| Chromosome: | chromosome 4 |
| Location: | 480597 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217924 | FAD5B | Fatty acid desaturase-like protein; (1 of 5) PTHR11351:SF2 - DELTA-9 ACYL-LIPID DESATURASE 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGTCTGTCGCCCACCCACTCAGTGCCCGCACCGGTTCTCTCTCCAA |
| Internal bar code: | TTTATCTTAGTAAGGCAGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2236 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGACACAGACGTGAGCT |
| Suggested primer 2: | ACCTCCAGCTCAAACTCGTG |